Coding

Part:BBa_K3783015

Designed by: Satvik Kethireddy   Group: iGEM21_OhioState   (2021-10-21)


LpxERF

Fusion of LpxE, LpxR, and LpxF with native Salmonella RBS between CDS of LpxE and LpxR; and LpxR and LpxF

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 768
    Illegal PstI site found at 1424
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 768
    Illegal PstI site found at 1424
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 768
    Illegal PstI site found at 1424
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 768
    Illegal PstI site found at 1424
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1858
    Illegal SapI.rc site found at 991


[edit]
Categories
Parameters
None